Agar from Red Algae (Gracilaria tenuistipitata) as a Valuable Biopolymer: Extraction and Characterization

Authors

  • Fawzia Afrin Rimpy Department of Biomedical Engineering, Military Institute of Science and Technology, Dhaka, Bangladesh
    • Md Enamul Hoque Military Institute of Science and Technology
      • Quazi Farsheed Mahmud Department of Biomedical Engineering, Military Institute of Science and Technology, Dhaka, Bangladesh
        • Itmam Nowroj Military Institute of Science and Technology
          • Sazedur Rahman Department of Mechanical, Aerospace, and Nuclear Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
            • Tarek El-Bialy Mike Petryk School of Dentistry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2E1, Canada
              • M. Azam Ali Ali University of Otago

                DOI:

                https://doi.org/10.47981/j.mijst.13(02)2025.536(27-38)

                Keywords:

                Agar; Red Algae; Gracilaria tenuistipitata; Cox’z Bazar; Biomedical Application

                Abstract

                Agar, a natural biopolymer extracted from red algae, holds immense potential for revolutionizing healthcare, including biomedical engineering. This study explores the feasibility of extracting agar from red algae (Gracilaria tenuistipitata) abundantly available in the coastal area of Cox’z Bazar, Bangladesh. Five extraction methods were investigated, including control and treatments with water and NaOH solutions at 2%, 4%, and 6% concentrations. Each method was applied to three extraction cycles, producing 15 samples for comprehensive analyses. The extracted agar samples were characterized through Fourier-transform infrared spectroscopy (FTIR), gel strength testing, melting and gelling temperature assessments, pH value measurement, and sulfate content analysis to determine their suitability for potential biomedical applications. Statistical tools such as ANOVA and Tukey's HSD test were employed to evaluate the influence of the pretreatment process on the yield and characteristics of agar. The results revealed significant variations across methods, emphasizing the critical role of extraction conditions in determining agar yield and characteristics. Among different alkali treatment methods, the sample processed with 2% NaOH and two hours treatment provided the highest agar yield of 11.67 %. Thus, two hours treatment with 2% NaOH was determined to be the optimal condition for agar extraction. This preliminary study suggests that the red algae is a promising source of agar for wider applications, including biomedical engineering. The agar extracted from abundant local sources in Bangladesh could unlock its potential for advancing healthcare solutions and sustainable national economic growth.

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                References

                A, A. D., & G, K. (2024). Microalgae: An emerging source of bioplastics production. Discover Environment, 2(1), 10. https://doi.org/10.1007/s44274-024-00038-0

                Armisén, R. (1991). Agar and agarose biotechnological applications. In J. A. Juanes, B. Santelices, & J. L. McLachlan (Eds.), International Workshop on Gelidium (pp. 157–166). Springer Netherlands. https://doi.org/10.1007/978-94-011-3610-5_15

                Beaumont, M., Tran, R., Vera, G., Niedrist, D., Rousset, A., Pierre, R., Shastri, V. P., & Forget, A. (2021). Hydrogel-Forming Algae Polysaccharides: From Seaweed to Biomedical Applications. Biomacromolecules, 22(3), 1027–1052. https://doi.org/10.1021/acs.biomac.0c01406

                Curvello, R., Raghuwanshi, V. S., & Garnier, G. (2019). Engineering nanocellulose hydrogels for biomedical applications. Advances in Colloid and Interface Science, 267, 47–61. https://doi.org/10.1016/j.cis.2019.03.002

                Dhivya, S., Padma, V. V., & Santhini, E. (2015). Wound dressings – a review. BioMedicine, 5(4), 22. https://doi.org/10.7603/s40681-015-0022-9

                El-Beltagi, H. S., Mohamed, A. A., Mohamed, H. I., Ramadan, K. M. A., Barqawi, A. A., & Mansour, A. T. (2022). Phytochemical and Potential Properties of Seaweeds and Their Recent Applications: A Review. Marine Drugs, 20(6), 342. https://doi.org/10.3390/md20060342

                Fujiwara, E., Rosa, L. O., Oku, H., & Cordeiro, C. M. B. (2023). Agar-based optical sensors for electric current measurements. Scientific Reports, 13(1), 13517. https://doi.org/10.1038/s41598-023-40749-7

                Gómez-Ordóñez, E., & Rupérez, P. (2011). FTIR-ATR spectroscopy as a tool for polysaccharide identification in edible brown and red seaweeds. Food Hydrocolloids, 25(6), 1514–1520. https://doi.org/10.1016/j.foodhyd.2011.02.009

                Graham, S., Marina, P. F., & Blencowe, A. (2019). Thermoresponsive polysaccharides and their thermoreversible physical hydrogel networks. Carbohydrate Polymers, 207, 143–159. https://doi.org/10.1016/j.carbpol.2018.11.053

                Hoque, M. E., Sarker, M. A., Arif, K., Ali, M. A., & El-Bialy, T. (2023). Antibacterial/Antiviral Face Masks: Processing, Characteristics, Challenges, and Sustainability. MIST INTERNATIONAL JOURNAL OF SCIENCE AND TECHNOLOGY, 11, 61–79. https://doi.org/10.47981/j.mijst.11(02)2023.421(61-79)

                Hossain, Md. T., Sohag, A. A. M., Haque, Md. N., Tahjib-Ul-Arif, Md., Dash, R., Chowdhury, M. T. H., Hossain, Md. A., Moon, I. S., & Hannan, Md. A. (2021). Nutritional Value, Phytochemical Profile, Antioxidant Property and Agar Yielding Potential of Macroalgae from Coasts of Cox’s Bazar and St. Martin’s Island of Bangladesh. Journal of Aquatic Food Product Technology, 30(2), 217–227. https://doi.org/10.1080/10498850.2020.1869876

                Jiang, F., Xu, X.-W., Chen, F.-Q., Weng, H.-F., Chen, J., Ru, Y., Xiao, Q., & Xiao, A.-F. (2023). Extraction, Modification and Biomedical Application of Agarose Hydrogels: A Review. Marine Drugs, 21(5), 299. https://doi.org/10.3390/md21050299

                Kazimierczak, P., Palka, K., & Przekora, A. (2019). Development and Optimization of the Novel Fabrication Method of Highly Macroporous Chitosan/Agarose/Nanohydroxyapatite Bone Scaffold for Potential Regenerative Medicine Applications. Biomolecules, 9(9), 434. https://doi.org/10.3390/biom9090434

                Khandwal, D., Patel, S., Pandey, A. K., & Mishra, A. (2025). A Comprehensive, Analytical Narrative Review of Polysaccharides from the Red Seaweed Gracilaria: Pharmaceutical Applications and Mechanistic Insights for Human Health. Nutrients, 17(5), 744. https://doi.org/10.3390/nu17050744

                Lam, P.-L., Gambari, R., Kok, S. H.-L., Lam, K.-H., Tang, J. C.-O., Bian, Z.-X., Lee, K. K.-H., & Chui, C.-H. (2015). Non-toxic agarose/gelatin-based microencapsulation system containing gallic acid for antifungal application. International Journal of Molecular Medicine, 35(2), 503–510. https://doi.org/10.3892/ijmm.2014.2027

                Liao, Y.-C., Chang, C.-C., Nagarajan, D., Chen, C.-Y., & Chang, J.-S. (2021). Algae-derived hydrocolloids in foods: Applications and health-related issues. Bioengineered, 12(1), 3787–3801. https://doi.org/10.1080/21655979.2021.1946359

                Madadi, R., Maljaee, H., Serafim, L. S., & Ventura, S. P. M. (2021). Microalgae as Contributors to Produce Biopolymers. Marine Drugs, 19(8), 466. https://doi.org/10.3390/md19080466

                Martínez-Sanz, M., Gómez-Mascaraque, L. G., Ballester, A. R., Martínez-Abad, A., Brodkorb, A., & López-Rubio, A. (2019). Production of unpurified agar-based extracts from red seaweed Gelidium sesquipedale by means of simplified extraction protocols. Algal Research, 38, 101420. https://doi.org/10.1016/j.algal.2019.101420

                Mohibbullah, Md., Talha, Md. A., Baten, Md. A., Newaz, A. W., & Choi, J. (2023). Yield optimization, physicochemical characterizations, and antioxidant properties of food grade agar from Gracilaria tenuistipitata of Cox’s Bazar coast, Bangladesh. Food Science & Nutrition, 11(6), 2852–2863. https://doi.org/10.1002/fsn3.3265

                Padmesh, S., & Singh, A. (2021). Agars: Properties and Applications. In Inamuddin, M. I. Ahamed, R. Boddula, & T. Altalhi (Eds.), Polysaccharides (1st ed., pp. 75–93). Wiley. https://doi.org/10.1002/9781119711414.ch5

                Park, S. H., Lee, C.-R., & Hong, S.-K. (2020). Implications of agar and agarase in industrial applications of sustainable marine biomass. Applied Microbiology and Biotechnology, 104(7), 2815–2832. https://doi.org/10.1007/s00253-020-10412-6

                Qari, R., & Haider, S. (2021). Agar Extraction, Physical Properties, FTIR Analysis and Biochemical Composition of Three Edible Species of Red Seaweeds Gracilaria corticata (J. Agardh), Gracilaria dentata (J. Agardh) and Gracilariopsis longissima (S. G. Gmelin)........ Biological Sciences - PJSIR, 64(3), 263–273. https://doi.org/10.52763/PJSIR.BIOL.SCI.64.3.2021.263.273

                Rahmati, M., Alipanahi, Z., & Mozafari, M. (2019). Emerging Biomedical Applications of Algal Polysaccharides. Current Pharmaceutical Design, 25(11), 1335–1344. https://doi.org/10.2174/1381612825666190423160357

                Rashid, A.B.; Hoque, M.E.; Kabir, N.; Rifat, F.F.; Ishrak, H.; Alqahtani, A.; Chowdhury, M.E.H. Synthesis, Properties, Applications, and Future Prospective of Cellulose Nanocrystals. Polymers 2023, 15, 4070. https://doi.org/10.3390/polym15204070

                Rhein-Knudsen, N., Ale, M. T., Ajalloueian, F., Yu, L., & Meyer, A. S. (2017). Rheological properties of agar and carrageenan from Ghanaian red seaweeds. Food Hydrocolloids, 63, 50–58. https://doi.org/10.1016/j.foodhyd.2016.08.023

                Sasuga, K., Yamanashi, T., Nakayama, S., Ono, S., & Mikami, K. (2017). Optimization of yield and quality of agar polysaccharide isolated from the marine red macroalga Pyropia yezoensis. Algal Research, 26, 123–130. https://doi.org/10.1016/j.algal.2017.07.010

                Sobuj, M. K. A., Islam, Md. A., Islam, Md. S., Islam, Md. M., Mahmud, Y., & Rafiquzzaman, S. M. (2021). Effect of solvents on bioactive compounds and antioxidant activity of Padina tetrastromatica and Gracilaria tenuistipitata seaweeds collected from Bangladesh. Scientific Reports, 11(1), 19082. https://doi.org/10.1038/s41598-021-98461-3

                Souza, B. W. S., Cerqueira, M. A., Bourbon, A. I., Pinheiro, A. C., Martins, J. T., Teixeira, J. A., Coimbra, M. A., & Vicente, A. A. (2012). Chemical characterization and antioxidant activity of sulfated polysaccharide from the red seaweed Gracilaria birdiae. Food Hydrocolloids, 27(2), 287–292. https://doi.org/10.1016/j.foodhyd.2011.10.005

                Sudhakar, M. P., Nived, S. A., & Dharani, G. (2024). Fabrication and Characterization of Agar‐ and Seaweed‐Derived Biomembrane Films for Biomedical and Other Applications. Biopolymers, e23643. https://doi.org/10.1002/bip.23643

                Sukhikh, S., Prosekov, A., Ivanova, S., Maslennikov, P., Andreeva, A., Budenkova, E., Kashirskikh, E., Tcibulnikova, A., Zemliakova, E., Samusev, I., & Babich, O. (2022). Identification of Metabolites with Antibacterial Activities by Analyzing the FTIR Spectra of Microalgae. Life, 12(9), 1395. https://doi.org/10.3390/life12091395

                Tatrishvili, T. (Ed.). (2025). Sustainability in Polymer Technology and Plastic Engineering: Concepts, Strategies, and Opportunities (First edition). Apple Academic Press.

                Thombare, N., Mahto, A., Singh, D., Chowdhury, A. R., & Ansari, M. F. (2023). Comparative FTIR Characterization of Various Natural Gums: A Criterion for Their Identification. Journal of Polymers and the Environment, 31(8), 3372–3380. https://doi.org/10.1007/s10924-023-02821-1

                Vieira, C. B., Sousa, J. R., Do Vale, D. A., Guimarães, C. P., De Sousa, K. C., Mattos, A. L. A., Silva, A. L. C., De Sá Moreira Souza Filho, M., & Souza, B. W. S. (2025). Edible films based on sulfated polysaccharides from the seaweed Gracilaria birdiae: Physicochemical, optical and mechanical properties. Carbohydrate Research, 552, 109473. https://doi.org/10.1016/j.carres.2025.109473

                Vijayan, S. R., Santhiyagu, P., Ramasamy, R., Arivalagan, P., Kumar, G., Ethiraj, K., & Ramaswamy, B. R. (2016). Seaweeds: A resource for marine bionanotechnology. Enzyme and Microbial Technology, 95, 45–57. https://doi.org/10.1016/j.enzmictec.2016.06.009

                Xiao, Q., Wang, X., Zhang, J., Zhang, Y., Chen, J., Chen, F., & Xiao, A. (2021). Pretreatment Techniques and Green Extraction Technologies for Agar from Gracilaria lemaneiformis. Marine Drugs, 19(11), 617. https://doi.org/10.3390/md19110617

                Zhang, C., An, D., Xiao, Q., Weng, H., Zhang, Y., Yang, Q., & Xiao, A. (2020). Preparation, characterization, and modification mechanism of agar treated with hydrogen peroxide at different temperatures. Food Hydrocolloids, 101, 105527. https://doi.org/10.1016/j.foodhyd.2019.105527

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                31-12-2025

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                How to Cite

                Rimpy, F. A., Hoque, M. E., Mahmud, Q. F., Nowroj, I., Rahman, S., El-Bialy, T., & Ali, M. A. A. (2025). Agar from Red Algae (Gracilaria tenuistipitata) as a Valuable Biopolymer: Extraction and Characterization. MIST INTERNATIONAL JOURNAL OF SCIENCE AND TECHNOLOGY, 13(2), 27-38. https://doi.org/10.47981/j.mijst.13(02)2025.536(27-38)

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