Visual Assessment and Genetic Distance Analysis of Gamma-Irradiated Lemon Leaf Mutants Developed Using the Leaf-Cut Method
DOI:
https://doi.org/10.47981/j.mijst.13(02)2025.559(65-71)Abstract
Ionizing radiation, particularly gamma rays, is widely used to induce genetic mutations for improving morphological traits and enhancing genetic diversity in plants. In citrus plants, such mutations can be achieved by irradiating leaves and propagating the resulting mutants through vegetative methods. This study was conducted at the BINA research farm with the objective of developing Citrus limon mutants using gamma radiation and analyzing their morphological characteristics through visual inspection, alongside genetic diversity assessment using hierarchical clustering. A total of 400 fresh leaves were collected from the mother plant (BINA Lebu-1) and exposed to different doses of gamma radiation (0, 60, 80, and 100 Gy), divided into four treatment batches. The irradiated samples were planted in unit plots following a Randomized Complete Block Design (RCBD). Root and shoot development were monitored visually after 3 to 4 months. Maximum root length was observed at 100 Gy, the highest root quantity at 60 Gy, and the highest success rate at 80 Gy.
After 7–8 months, mature plants were further assessed, and leaves were collected for genomic analysis. Genetic relationships among the different treatment groups were evaluated using a Dendrogram generated through simple hierarchical clustering. The results indicated clear genetic dissimilarities between irradiated (60, 80, and 100 Gy) and non-irradiated (0 Gy) plants, with the 100 Gy group showing the greatest genetic distance from the others.
Due to time constraints, fruit production could not be observed. Nonetheless, the study demonstrates the effectiveness of combining visual inspection and genetic distance analysis in evaluating gamma-induced citrus mutants developed via the leaf-cut method.
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References
Barkley, N.A.; Roose, M.L.; Krueger, R.R. & Federici, C.T. (2006). Assessing genetic diversity and population structure in a citrus germplasm collection utilizing simple sequence repeat markers (SSRs). Theor. Appl. Genet. 112, 1519–1531.
Belele C.L., Vieira G.S. and Goulart L.R. (2001). Effects of gamma radiation of morphological traits and seed storage proteins of bean, Legume Research, 45, 23
Çimen, B., Yeşiloğlu, T., & Aka Kaçar, Y. (2020). Effects of Gamma Irradiation on Seedlessness and Fruit Quality of Ortanique Tangor. Turkish Journal of Agriculture - Food Science and Technology
Deependra Yadav and S.P. Singh. (2018). Vegetative methods of plant propagation: I- cutting layering and budding. Journal of Pharmacognosy and Phytochemistry 7(2) 3267- 3273
Gulsen, O. & Roose, M.L. (2001). Lemons: diversity and relationships with selected citrus genotypes as measured with nuclear genome markers. J. Am. Soc. Hort. Sci. 126: 309–317.
Hodgson, R. W. (1967). Horticultural varieties of Citrus. The Citrus Industry (W. Reuther, H. J. Webber, and L. D. Batchelor. eds.). University of California Press. Berkely, 1: 431-591.
Kumar, S., Kumari, R., & Kumar, S. (2013). Plant breeding by mutation induction: Current trends. Botany Research International, 6(2), 41–48
Sidler, C., Li, D. P., Kovalchuk, O., and Kovalchuk, I. (2015). Development dependent expression of DNA repair genes and epigenetic regulators in Arabidopsis plants exposed to ionizing radiation. Radiat. Res. 183, 219–232.
Sparrow, A.H. and Gunckel, J.E. 1956. The effect of plants on chronic exposure to gamma-radiation from radio cobalt. Proc Int Conf Peaceful Uses of Atomic Energy, 17:52-59
Taheri, S., Abdullah, T. L., Ahmad, Z., and Abdullah, N. A. P. (2014). Effect of acute gamma irradiation on Curcuma alismatifolia varieties and detection of DNA polymorphism through SSR Marker. Biomed Res. Int. 2014:631813.
Waqar, A., Wasim, A. Farooqi and Sattar (Jr), A. (1992). Effect of gamma irradiation on the morphology of Kinnow seedlings. Proc First Intl. Seminar on Citriculture in Pakistan, 2-5 Dec, 1992
Whittwer, S.H. (1971). Radiation induced mutation in crop plants. Outlook Agri., 6:205-17
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