Prevalence of Multidrug-Resistant Salmonella in Raw Salad Vegetables in Dhaka Metropolitan Area

Palash Kumar Sarker; Hafizur Rahman; Kulsum Ara; Md. Shahidul Kabir; Shishir Kanti  Pramanik; Maruf  Hasan

doi:10.47981/j.mijst.11(01)2023.396(51-57)

Palash Kumar Sarker Department of Science and Humanities, Military Institute of Science and Technology (MIST), Dhaka, Bangladesh
Hafizur Rahman Department of Environmental Science and Management, Independent University, Bangladesh (IUB), Dhaka, Bangladesh
Kulsum Ara Institute of Public Health (IPH), Dhaka, Bangladesh
Md. Shahidul Kabir Department of Microbiology, Stamford University Bangladesh, Dhaka, Bangladesh
Shishir Kanti Pramanik Department of Chemistry, Graduate School of Physical Sciences, Shahjalal University of Science and Technology, Sylhet, Bangladesh
Maruf Hasan Department of Biomedical Engineering, Military Institute of Science and Technology (MIST), Dhaka, Bangladesh

DOI: https://doi.org/10.47981/j.mijst.11(01)2023.396(51-57)

Keywords: Vegetable salad, Multidrug-resistant (MDR), Pathogens, Fecal coliform, E. coli

Abstract

Raw salad vegetables have become very popular among consumers due to their multiple health benefits. The bacteriological quality of these salad vegetables is of great public health concern. This study was conducted to determine the presence of potential pathogenic and multidrug-resistant (MDR) bacteria in raw salad vegetables served in different restaurants in Dhaka, the capital city of Bangladesh. A total of 50 samples, comprising different types of raw salad vegetables were collected from restaurants in Mirpur (n=10), Dhanmondi (n=10), Old Dhaka (n=10), Gulshan (n=10) and Bashundhara Residential Area (n=10). The highest counts for total coliforms and fecal coliforms were 4.02 × 10³ cfu/ml and 1.3×10³ cfu/ml, respectively. The isolates were E. coli, total coliform, fecal coliform and Salmonella spp. Among them, fecal coliform and Salmonella spp. were found to be more pathogenic. As potential pathogenic bacteria, Salmonella spp. were tested for antibiotic sensitivity and all of them showed resistance against amoxicillin, ampicillin, azithromycin, chloramphenicol, ciprofloxacin, erythromycin, kanamycin, streptomycin, tetracycline, norfloxacin, and trimethoprim. The findings assist to understand the level of contamination of pathogenic and MDR bacteria in raw salad vegetables, which will create awareness of food safety and public health.

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References

Akhie, A. A., & Ahmed, A. (2018, March). Water Supply and Sanitation Scarcity Assessment of Urban Slum: A Comprehensive Case Study for Mirpur. In Proceedings of the 1st National Conference on Water Resources Engineering (NCWRE 2018) (Vol. 21, p. 22).

Akoachere, J. F. T. K., Tatsinkou, B. F., & Nkengfack, J. M. (2018). Bacterial and parasitic contaminants of salad vegetables sold in markets in Fako Division, Cameroon and evaluation of hygiene and handling practices of vendors. BMC research notes, 11(1), 1-7. https://doi.org/10.1186/s13104-018-3175-2

Alam, M. S., Feroz, F., Rahman, H., Das, K. K., & Noor, R. (2015). Microbiological contamination sources of freshly cultivated vegetables. Nutrition & Food Science, 45(4), 646-658. https://doi.org/10.1108/NFS-04-2015-0032

Alam, M., Halder, A., & Horng, L. (2017). Bangladesh national hygiene baseline survey preliminary report. Dhaka, Bangladesh2014.

Alfrad, E. B. (2007), Bensons Microbiological Applications, Mcgraw-Hill Book Company, NewYork, NY, pp. 263-280

Amaechi, E. C., Ohaeri, C. C., Ukpai, O. M., & Adegbite, R. A. (2016). Prevalence of parasitic contamination of salad vegetables in Ilorin, North Central, Nigeria. Momona Ethiopian Journal of Science, 8(2), 136-145. https://doi.org/10.4314/mejs.v8i2.3

Ali, M., Khan, M. R., & Saha, M. L. (2011). Antibiotic resistant patterns of bacterial isolates from ready-to-eat (RTE) street vended fresh vegetables and fruits in Dhaka City. Bangladesh Journal of Scientific Research, 24(2), 127-134.

Asaduzzaman, M. , & Asao, T. , (Eds.). (2018). Vegetables - Importance of Quality Vegetables to Human Health. IntechOpen. https://doi.org/10.5772/intechopen.70972

Bangladesh Bureau of Statistics, Statistics and Informatics Division, Ministry of Planning. (2012). Community Report, Dhaka Zila, Population and Housing Census 2011.

Brookie, K. L., Best, G. I., & Conner, T. S. (2018). Intake of raw fruits and vegetables is associated with better mental health than intake of processed fruits and vegetables. Frontiers in psychology, 9, 487. https://doi.org/10.3389/fpsyg.2018.00487

Cappuccino, G. J., & Sherman, N. D. (1996). Microbiology: A laboratory manual 5 th ed the Benjamin.

Cardamone, C., Aleo, A., Mammina, C., Oliveri, G., & Di Noto, A. M. (2015). Assessment of the microbiological quality of fresh produce on sale in Sicily, Italy: preliminary results. Journal of biological research (Thessalonike, Greece), 22(1), 3 .https://doi.org/10.1186/s40709-015-0026-3

Chau, H. L. Q., Thong, H. T., Chao, N. V., Hung, P. H. S., Hai, V. V., An, L. V., ... & Akamatsu, M. (2014). Microbial and parasitic contamination on fresh vegetables sold in traditional markets in Hue City, Vietnam. J Food Nutr Res, 2(12), 959-964. https://doi.org/10.12691/jfnr-2-12-16

Cruz, M. R. G. D., Leite, Y. J. B. D. S., Marques, J. D. L., Pavelquesi, S. L. S., Oliveira, L. R. D. A., Silva, I. C. R. D., & Orsi, D. C. (2019). Microbiological quality of minimally processed vegetables commercialized in Brasilia, DF, Brazil. Food Science and Technology, 39, 498-503. https://doi.org/10.1590/fst.16018

Feroz, F., Senjuti, J. D., & Noor, R. (2013). Determination of microbial growth and survival in salad vegetables through in vitro challenge test. International Journal of Nutrition and Food Sciences, 2(6), 312-319.

Frank, S. M., Webster, J., McKenzie, B., Geldsetzer, P., Manne-Goehler, J., Andall-Brereton, G., Houehanou, C., Houinato, D., Gurung, M. S., Bicaba, B. W., McClure, R. W., Supiyev, A., Zhumadilov, Z., Stokes, A., Labadarios, D., Sibai, A. M., Norov, B., Aryal, K. K., Karki, K. B., Kagaruki, G. B., … Jaacks, L. M. (2019). Consumption of Fruits and Vegetables Among Individuals 15 Years and Older in 28 Low- and Middle-Income Countries. The Journal of nutrition, 149(7), 1252–1259. https://doi.org/10.1093/jn/nxz040

Hasan, M. K., Shahriar, A., & Jim, K. U. (2019). Water pollution in Bangladesh and its impact on public health. Heliyon, 5(8), e02145. https://doi.org/10.1016/j.heliyon.2019.e02145

IU, N., VC, E., Ce, O., & Ju, F. (2015). Evaluation of the degree of contamination of salad vegetables sold in Umuahia Main Market. American Journal of Microbiological Research, 3(1), 41-44.

Jorgensen, J. H., & Pfaller, M. A. (2015). Introduction to the 11th Edition of the Manual of Clinical Microbiology. Manual of Clinical Microbiology, 1-4. https://doi.org/10.1128/9781555817381.ch1

Kamruzzaman, M., & Ogura, N. (2007). Apartment housing in Dhaka City: Past, present and characteristic outlook. Building Stock Activation, Tokyo, Japan.

Khairuzzaman, M., Chowdhury, F. M., Zaman, S., Al Mamun, A., & Bari, M. L. (2014). Food Safety Challenges towards Safe, Healthy, and Nutritious Street Foods in Bangladesh. International journal of food science, 2014, 483519. https://doi.org/10.1155/2014/483519

Kim, S., Frye, J. G., Hu, J., Fedorka-Cray, P. J., Gautom, R., & Boyle, D. S. (2006). Multiplex PCR-based method for identification of common clinical serotypes of Salmonella enterica subsp. enterica. Journal of clinical microbiology, 44(10), 3608–3615. https://doi.org/10.1128/JCM.00701-06

Liu R. H. (2013). Health-promoting components of fruits and vegetables in the diet. Advances in nutrition (Bethesda, Md.), 4(3), 384S–92S. https://doi.org/10.3945/an.112.003517

Luna-Guevara, J. J., Arenas-Hernandez, M. M. P., Martínez de la Peña, C., Silva, J. L., & Luna-Guevara, M. L. (2019). The Role of Pathogenic E. coli in Fresh Vegetables: Behavior, Contamination Factors, and Preventive Measures. International journal of microbiology, 2019, 2894328. https://doi.org/10.1155/2019/2894328

Mohamed, M. A., Siddig, E. E., Elaagip, A. H., Edris, A. M. M., & Nasr, A. A. (2016). Parasitic contamination of fresh vegetables sold at central markets in Khartoum state, Sudan. Annals of Clinical Microbiology and Antimicrobials, 15(1), 1-7. https://doi.org/10.1186/s12941-016-0133-5

Moura, S. C. S. R. D., & Vialta, A. (2022). use of fruits and vegetables in processed foods: consumption trends and technological impacts. Food Science and Technology, 42. https://doi.org/10.1590/fst.66421

Nizame, F. A., Alam, M. U., Masud, A. A., Shoab, A. K., Opel, A., Islam, M. K., Luby, S. P., & Unicomb, L. (2019). Hygiene in Restaurants and among Street Food Vendors in Bangladesh. The American journal of tropical medicine and hygiene, 101(3), 566–575. https://doi.org/10.4269/ajtmh.18-0896

Noor, R., & Feroz, F. (2016). Food safety in Bangladesh: A microbiological perspective. Stamford Journal of Microbiology, 6(1), 1-6. https://doi.org/10.3329/sjm.v6i1.33509

Noor, R., Malek, M., Rahman, S., Meghla, M., Acharjee, M., & Rahman, M. M. (2015). Assessment of survival of pathogenic bacteria in fresh vegetables through in vitro challenge test. International Journal of Food Contamination, 2(1), 1-9.

Rahman, F., & Noor, R. (2012). Prevalence of pathogenic bacteria in common salad vegetables of Dhaka Metropolis. Bangladesh Journal of Botany, 41(2), 159-162.

Rozario, S. R., Rahman, H., Fakhruddin, A. N. M., & Rabbani, K. A. (2019). Prevalence of Multidrug-Resistant Bacteria on Mobile Phone Surface. Journal of microscopy and ultrastructure, 8(1), 14–19. https://doi.org/10.4103/JMAU.JMAU_7_19

Samtiya, M., Aluko, R. E., Dhewa, T., & Moreno-Rojas, J. M. (2021). Potential Health Benefits of Plant Food-Derived Bioactive Components: An Overview. Foods (Basel, Switzerland), 10(4), 839. https://doi.org/10.3390/foods10040839

Satu, S. A., & Chiu, R. L. (2019). Livability in dense residential neighbourhoods of Dhaka. Housing Studies, 34(3), 538-559. https://doi.org/10.1080/02673037.2017.1364711

Slavin, J. L., & Lloyd, B. (2012). Health benefits of fruits and vegetables. Advances in nutrition (Bethesda, Md.), 3(4), 506–516. https://doi.org/10.3945/an.112.002154

Tanwar, J., Das, S., Fatima, Z., & Hameed, S. (2014). Multidrug resistance: an emerging crisis. Interdisciplinary perspectives on infectious diseases, 2014, 541340. https://doi.org/10.1155/2014/541340

Tefera, T., Biruksew, A., Mekonnen, Z., & Eshetu, T. (2014). Parasitic Contamination of Fruits and Vegetables Collected from Selected Local Markets of Jimma Town, Southwest Ethiopia. International scholarly research notices, 2014, 382715. https://doi.org/10.1155/2014/382715

Wayne, P. A. (2020). Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing.